Cloning

In earlier days, production of industrially important enzymes was carried out by conventional methods of optimizing cultural conditions in flasks and fermentors. With passage of time new techniques are being developed like cloning. In cloning specific genes are amplified and ligated in specific plasmid that is in turn transformed into the host cells. In this book cloning and expression of alpha amylase gene of Bacillus licheniformis is discussed. pTZ57R/T and pET22b plasmids are used to clone amylase gene. Reasonable amount of amylase is obtained. By using this technique large amount of enzymes are produced with less effort as compared to conventional fermentation methods. This book could be helpful for students and teachers in the subjects of biology, biochemistry, biotechnology, genetics and molecular biology.